MetaPointFinder

Many clinically important antimicrobial resistance (AMR) phenotypes such as fluoroquinolones and rifamycins are driven by antimicrobial resistance-conferring mutations (ARMs) in conserved chromosomal loci (e.g., gyrA, parC, rpoB).

Resistome profiling by metagenomic sequencing is ideal for AMR surveillance, but existing pipelines detect only acquired genes and ignore point mutations. MetaPointFinder bridges this gap.

The tool identifies reads carrying resistance-determining regions using DIAMOND (protein) and KMA (DNA), aligns them, and classifies resistant versus wild-type variants using known mutations from the AMRFinderPlus database.

This web version allows you to test MetaPointFinder. For large datasets, please install the full version on a local Linux machine.

The repository of MetaPointFinder is available here: https://github.com/aldertzomer/metapointfinder

The input should be a single .fastq.gz file containing your reads.

The cutoff defines the minimal identity used when matching reads to AMR reference genes.