SUMMARISING RUN PARAMETERS ========================== Input filename: ERR3932995_1.fastq Trimming mode: paired-end Trim Galore version: 0.6.4_dev Cutadapt version: 3.5 Number of cores used for trimming: 1 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Nextera adapter for trimming (count: 2365). Second best hit was smallRNA (count: 0) Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 100 bp This is cutadapt 3.5 with Python 3.10.12 Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA ERR3932995_1.fastq Processing reads on 1 core in single-end mode ... Finished in 9.59 s (11 µs/read; 5.56 M reads/minute). === Summary === Total reads processed: 889,123 Reads with adapters: 266,299 (30.0%) Reads written (passing filters): 889,123 (100.0%) Total basepairs processed: 169,087,125 bp Quality-trimmed: 1,029,351 bp (0.6%) Total written (filtered): 167,397,303 bp (99.0%) === Adapter 1 === Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 266299 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-12 bp: 1 Bases preceding removed adapters: A: 44.2% C: 16.2% G: 14.6% T: 25.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 228024 222280.8 0 228024 2 22402 55570.2 0 22402 3 5741 13892.5 0 5741 4 1572 3473.1 0 1572 5 444 868.3 0 444 6 242 217.1 0 242 7 39 54.3 0 39 8 78 13.6 0 78 9 119 3.4 0 109 10 10 87 0.8 1 38 49 11 247 0.2 1 110 137 12 252 0.1 1 97 155 13 201 0.1 1 68 133 14 156 0.1 1 49 107 15 133 0.1 1 33 100 16 99 0.1 1 21 78 17 229 0.1 1 61 168 18 77 0.1 1 18 59 19 87 0.1 1 22 65 20 128 0.1 1 32 96 21 154 0.1 1 39 115 22 96 0.1 1 23 73 23 140 0.1 1 49 91 24 93 0.1 1 30 63 25 97 0.1 1 27 70 26 126 0.1 1 39 87 27 96 0.1 1 30 66 28 125 0.1 1 34 91 29 114 0.1 1 41 73 30 107 0.1 1 28 79 31 105 0.1 1 30 75 32 61 0.1 1 14 47 33 89 0.1 1 30 59 34 110 0.1 1 23 87 35 86 0.1 1 28 58 36 123 0.1 1 32 91 37 113 0.1 1 37 76 38 135 0.1 1 38 97 39 65 0.1 1 21 44 40 98 0.1 1 36 62 41 146 0.1 1 50 96 42 125 0.1 1 38 87 43 142 0.1 1 33 109 44 59 0.1 1 16 43 45 22 0.1 1 6 16 46 34 0.1 1 10 24 47 42 0.1 1 8 34 48 45 0.1 1 12 33 49 71 0.1 1 29 42 50 57 0.1 1 19 38 51 91 0.1 1 26 65 52 77 0.1 1 24 53 53 90 0.1 1 21 69 54 91 0.1 1 29 62 55 94 0.1 1 25 69 56 104 0.1 1 32 72 57 49 0.1 1 14 35 58 64 0.1 1 15 49 59 73 0.1 1 24 49 60 53 0.1 1 14 39 61 43 0.1 1 10 33 62 66 0.1 1 17 49 63 46 0.1 1 9 37 64 86 0.1 1 28 58 65 81 0.1 1 20 61 66 80 0.1 1 23 57 67 47 0.1 1 10 37 68 66 0.1 1 19 47 69 86 0.1 1 19 67 70 76 0.1 1 19 57 71 83 0.1 1 20 63 72 114 0.1 1 36 78 73 108 0.1 1 33 75 74 140 0.1 1 52 88 75 168 0.1 1 71 97 76 200 0.1 1 67 133 77 128 0.1 1 35 93 78 78 0.1 1 32 46 79 28 0.1 1 7 21 80 26 0.1 1 6 20 81 11 0.1 1 2 9 82 11 0.1 1 2 9 83 20 0.1 1 6 14 84 12 0.1 1 2 10 85 13 0.1 1 4 9 86 9 0.1 1 4 5 87 9 0.1 1 2 7 88 11 0.1 1 2 9 89 12 0.1 1 4 8 90 4 0.1 1 2 2 91 9 0.1 1 2 7 92 8 0.1 1 1 7 93 7 0.1 1 3 4 94 6 0.1 1 0 6 95 7 0.1 1 1 6 96 8 0.1 1 3 5 97 3 0.1 1 0 3 98 1 0.1 1 0 1 99 4 0.1 1 2 2 100 7 0.1 1 2 5 101 6 0.1 1 0 6 102 7 0.1 1 2 5 103 5 0.1 1 1 4 104 6 0.1 1 0 6 105 6 0.1 1 0 6 106 7 0.1 1 1 6 107 4 0.1 1 1 3 108 5 0.1 1 3 2 109 2 0.1 1 0 2 110 6 0.1 1 1 5 111 7 0.1 1 0 7 112 3 0.1 1 1 2 113 7 0.1 1 0 7 114 2 0.1 1 0 2 115 3 0.1 1 1 2 116 3 0.1 1 1 2 117 3 0.1 1 1 2 118 3 0.1 1 0 3 119 5 0.1 1 2 3 120 5 0.1 1 0 5 121 4 0.1 1 1 3 122 3 0.1 1 1 2 123 3 0.1 1 0 3 124 5 0.1 1 2 3 125 5 0.1 1 0 5 126 2 0.1 1 0 2 127 1 0.1 1 0 1 128 4 0.1 1 1 3 129 3 0.1 1 1 2 130 7 0.1 1 4 3 131 4 0.1 1 1 3 132 3 0.1 1 0 3 133 8 0.1 1 1 7 134 6 0.1 1 0 6 135 3 0.1 1 0 3 136 7 0.1 1 1 6 137 3 0.1 1 1 2 138 6 0.1 1 0 6 139 1 0.1 1 0 1 140 3 0.1 1 2 1 141 4 0.1 1 2 2 142 4 0.1 1 0 4 143 2 0.1 1 1 1 144 2 0.1 1 0 2 145 5 0.1 1 0 5 146 3 0.1 1 0 3 147 1 0.1 1 0 1 148 3 0.1 1 0 3 149 3 0.1 1 0 3 150 2 0.1 1 0 2 151 2 0.1 1 0 2 152 3 0.1 1 1 2 153 4 0.1 1 0 4 154 3 0.1 1 1 2 155 3 0.1 1 0 3 156 2 0.1 1 0 2 157 2 0.1 1 0 2 158 4 0.1 1 0 4 159 2 0.1 1 1 1 160 3 0.1 1 0 3 161 2 0.1 1 0 2 162 2 0.1 1 0 2 163 1 0.1 1 0 1 164 1 0.1 1 0 1 165 2 0.1 1 0 2 166 3 0.1 1 1 2 167 6 0.1 1 0 6 168 5 0.1 1 0 5 169 1 0.1 1 1 170 1 0.1 1 0 1 171 3 0.1 1 0 3 173 2 0.1 1 0 2 174 7 0.1 1 0 7 175 2 0.1 1 0 2 176 1 0.1 1 0 1 177 3 0.1 1 0 3 178 6 0.1 1 2 4 180 2 0.1 1 0 2 181 6 0.1 1 2 4 183 3 0.1 1 1 2 184 1 0.1 1 0 1 185 1 0.1 1 0 1 186 2 0.1 1 0 2 188 1 0.1 1 0 1 189 4 0.1 1 0 4 190 4 0.1 1 1 3 191 1 0.1 1 0 1 193 3 0.1 1 0 3 194 4 0.1 1 2 2 195 3 0.1 1 0 3 196 1 0.1 1 0 1 198 2 0.1 1 0 2 200 1 0.1 1 0 1 201 5 0.1 1 0 5 202 1 0.1 1 0 1 203 2 0.1 1 1 1 204 1 0.1 1 0 1 205 3 0.1 1 0 3 206 1 0.1 1 0 1 207 1 0.1 1 0 1 208 2 0.1 1 0 2 214 1 0.1 1 0 1 216 1 0.1 1 0 1 217 1 0.1 1 0 1 220 1 0.1 1 0 1 223 1 0.1 1 0 1 226 4 0.1 1 0 4 227 2 0.1 1 0 2 228 2 0.1 1 0 2 233 2 0.1 1 0 2 234 1 0.1 1 0 1 236 1 0.1 1 0 1 237 1 0.1 1 0 1 241 1 0.1 1 0 1 242 1 0.1 1 0 1 243 2 0.1 1 0 2 RUN STATISTICS FOR INPUT FILE: ERR3932995_1.fastq ============================================= 889123 sequences processed in total