SUMMARISING RUN PARAMETERS ========================== Input filename: ERR3932984_1.fastq Trimming mode: paired-end Trim Galore version: 0.6.4_dev Cutadapt version: 3.5 Number of cores used for trimming: 1 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Nextera adapter for trimming (count: 825). Second best hit was Illumina (count: 0) Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 100 bp This is cutadapt 3.5 with Python 3.10.12 Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA ERR3932984_1.fastq Processing reads on 1 core in single-end mode ... Finished in 4.84 s (11 µs/read; 5.56 M reads/minute). === Summary === Total reads processed: 448,631 Reads with adapters: 138,439 (30.9%) Reads written (passing filters): 448,631 (100.0%) Total basepairs processed: 76,249,761 bp Quality-trimmed: 376,287 bp (0.5%) Total written (filtered): 75,540,176 bp (99.1%) === Adapter 1 === Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 138439 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-12 bp: 1 Bases preceding removed adapters: A: 44.6% C: 16.3% G: 14.6% T: 24.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 121273 112157.8 0 121273 2 10793 28039.4 0 10793 3 2759 7009.9 0 2759 4 561 1752.5 0 561 5 176 438.1 0 176 6 67 109.5 0 67 7 13 27.4 0 13 8 15 6.8 0 15 9 40 1.7 0 26 14 10 23 0.4 1 4 19 11 25 0.1 1 5 20 12 44 0.0 1 14 30 13 37 0.0 1 8 29 14 40 0.0 1 11 29 15 25 0.0 1 9 16 16 17 0.0 1 3 14 17 36 0.0 1 11 25 18 25 0.0 1 4 21 19 13 0.0 1 1 12 20 22 0.0 1 5 17 21 31 0.0 1 12 19 22 10 0.0 1 2 8 23 29 0.0 1 13 16 24 18 0.0 1 6 12 25 24 0.0 1 7 17 26 27 0.0 1 11 16 27 17 0.0 1 4 13 28 24 0.0 1 9 15 29 42 0.0 1 12 30 30 22 0.0 1 4 18 31 32 0.0 1 14 18 32 17 0.0 1 1 16 33 20 0.0 1 5 15 34 22 0.0 1 9 13 35 21 0.0 1 5 16 36 31 0.0 1 10 21 37 29 0.0 1 5 24 38 29 0.0 1 3 26 39 19 0.0 1 9 10 40 19 0.0 1 8 11 41 30 0.0 1 7 23 42 44 0.0 1 10 34 43 40 0.0 1 11 29 44 21 0.0 1 4 17 45 17 0.0 1 4 13 46 10 0.0 1 4 6 47 17 0.0 1 6 11 48 12 0.0 1 5 7 49 27 0.0 1 10 17 50 18 0.0 1 5 13 51 36 0.0 1 9 27 52 17 0.0 1 3 14 53 31 0.0 1 9 22 54 29 0.0 1 7 22 55 26 0.0 1 9 17 56 26 0.0 1 8 18 57 21 0.0 1 5 16 58 25 0.0 1 10 15 59 30 0.0 1 10 20 60 23 0.0 1 4 19 61 21 0.0 1 8 13 62 37 0.0 1 9 28 63 20 0.0 1 4 16 64 21 0.0 1 8 13 65 36 0.0 1 10 26 66 26 0.0 1 11 15 67 25 0.0 1 8 17 68 23 0.0 1 6 17 69 14 0.0 1 4 10 70 40 0.0 1 13 27 71 27 0.0 1 7 20 72 39 0.0 1 16 23 73 57 0.0 1 15 42 74 81 0.0 1 25 56 75 110 0.0 1 45 65 76 150 0.0 1 55 95 77 102 0.0 1 32 70 78 66 0.0 1 23 43 79 37 0.0 1 9 28 80 25 0.0 1 7 18 81 22 0.0 1 9 13 82 13 0.0 1 3 10 83 15 0.0 1 6 9 84 10 0.0 1 6 4 85 9 0.0 1 2 7 86 5 0.0 1 1 4 87 6 0.0 1 3 3 88 12 0.0 1 2 10 89 8 0.0 1 4 4 90 6 0.0 1 1 5 91 6 0.0 1 1 5 92 8 0.0 1 1 7 93 4 0.0 1 0 4 94 4 0.0 1 0 4 95 7 0.0 1 3 4 96 5 0.0 1 0 5 97 8 0.0 1 1 7 98 5 0.0 1 1 4 99 10 0.0 1 1 9 100 8 0.0 1 6 2 102 4 0.0 1 0 4 103 3 0.0 1 1 2 104 4 0.0 1 1 3 105 5 0.0 1 0 5 106 1 0.0 1 0 1 107 2 0.0 1 0 2 108 2 0.0 1 0 2 109 1 0.0 1 0 1 110 1 0.0 1 0 1 111 7 0.0 1 3 4 112 7 0.0 1 2 5 113 5 0.0 1 0 5 114 6 0.0 1 0 6 115 3 0.0 1 1 2 116 5 0.0 1 0 5 117 2 0.0 1 0 2 118 5 0.0 1 1 4 119 3 0.0 1 1 2 120 6 0.0 1 3 3 121 6 0.0 1 1 5 122 6 0.0 1 1 5 123 3 0.0 1 0 3 124 9 0.0 1 0 9 125 7 0.0 1 1 6 126 6 0.0 1 0 6 127 2 0.0 1 1 1 128 4 0.0 1 1 3 129 4 0.0 1 2 2 130 3 0.0 1 1 2 131 3 0.0 1 1 2 132 3 0.0 1 1 2 133 5 0.0 1 1 4 134 7 0.0 1 2 5 135 4 0.0 1 1 3 136 7 0.0 1 2 5 137 2 0.0 1 1 1 138 1 0.0 1 0 1 139 6 0.0 1 3 3 140 2 0.0 1 1 1 141 3 0.0 1 0 3 142 2 0.0 1 0 2 143 4 0.0 1 1 3 144 2 0.0 1 0 2 145 4 0.0 1 1 3 146 1 0.0 1 0 1 147 2 0.0 1 0 2 148 3 0.0 1 0 3 149 2 0.0 1 0 2 150 1 0.0 1 0 1 151 2 0.0 1 0 2 152 2 0.0 1 0 2 154 3 0.0 1 0 3 155 3 0.0 1 2 1 156 2 0.0 1 0 2 157 2 0.0 1 1 1 158 3 0.0 1 1 2 159 4 0.0 1 1 3 160 2 0.0 1 0 2 161 1 0.0 1 0 1 162 3 0.0 1 2 1 163 4 0.0 1 1 3 164 1 0.0 1 1 165 3 0.0 1 0 3 166 2 0.0 1 0 2 168 2 0.0 1 0 2 169 3 0.0 1 1 2 170 3 0.0 1 2 1 171 4 0.0 1 1 3 172 1 0.0 1 0 1 173 5 0.0 1 1 4 175 3 0.0 1 1 2 176 1 0.0 1 0 1 177 3 0.0 1 1 2 178 5 0.0 1 0 5 180 2 0.0 1 0 2 181 3 0.0 1 1 2 182 1 0.0 1 0 1 183 3 0.0 1 1 2 185 4 0.0 1 1 3 187 2 0.0 1 1 1 188 2 0.0 1 2 189 2 0.0 1 0 2 191 3 0.0 1 2 1 193 4 0.0 1 1 3 194 1 0.0 1 0 1 195 3 0.0 1 3 196 2 0.0 1 1 1 198 1 0.0 1 0 1 199 1 0.0 1 0 1 200 4 0.0 1 0 4 203 1 0.0 1 0 1 206 1 0.0 1 0 1 207 4 0.0 1 0 4 208 2 0.0 1 0 2 209 3 0.0 1 1 2 212 1 0.0 1 0 1 213 1 0.0 1 1 214 2 0.0 1 1 1 216 1 0.0 1 1 218 1 0.0 1 0 1 219 1 0.0 1 0 1 223 1 0.0 1 0 1 224 1 0.0 1 0 1 225 2 0.0 1 0 2 229 1 0.0 1 0 1 234 1 0.0 1 0 1 237 2 0.0 1 0 2 241 2 0.0 1 0 2 243 3 0.0 1 0 3 247 1 0.0 1 0 1 250 1 0.0 1 0 1 RUN STATISTICS FOR INPUT FILE: ERR3932984_1.fastq ============================================= 448631 sequences processed in total