SUMMARISING RUN PARAMETERS ========================== Input filename: ERR3932951_1.fastq Trimming mode: paired-end Trim Galore version: 0.6.4_dev Cutadapt version: 3.5 Number of cores used for trimming: 1 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Nextera adapter for trimming (count: 63). Second best hit was smallRNA (count: 0) Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 100 bp This is cutadapt 3.5 with Python 3.10.12 Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA ERR3932951_1.fastq Processing reads on 1 core in single-end mode ... Finished in 2.36 s (12 µs/read; 5.15 M reads/minute). === Summary === Total reads processed: 202,812 Reads with adapters: 34,948 (17.2%) Reads written (passing filters): 202,812 (100.0%) Total basepairs processed: 46,751,650 bp Quality-trimmed: 175,851 bp (0.4%) Total written (filtered): 46,510,121 bp (99.5%) === Adapter 1 === Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 34948 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-12 bp: 1 Bases preceding removed adapters: A: 37.8% C: 18.2% G: 15.1% T: 28.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 29017 50703.0 0 29017 2 4236 12675.8 0 4236 3 1184 3168.9 0 1184 4 172 792.2 0 172 5 41 198.1 0 41 6 16 49.5 0 16 7 1 12.4 0 1 8 1 3.1 0 1 9 3 0.8 0 1 2 10 3 0.2 1 0 3 11 8 0.0 1 1 7 12 3 0.0 1 0 3 13 2 0.0 1 0 2 14 3 0.0 1 1 2 15 5 0.0 1 1 4 16 5 0.0 1 0 5 17 4 0.0 1 0 4 18 4 0.0 1 1 3 19 2 0.0 1 1 1 20 1 0.0 1 0 1 21 3 0.0 1 1 2 22 1 0.0 1 0 1 23 3 0.0 1 0 3 24 3 0.0 1 2 1 25 3 0.0 1 2 1 26 4 0.0 1 0 4 28 3 0.0 1 1 2 29 2 0.0 1 0 2 30 3 0.0 1 1 2 31 4 0.0 1 0 4 33 3 0.0 1 0 3 34 3 0.0 1 1 2 35 4 0.0 1 0 4 37 2 0.0 1 0 2 39 1 0.0 1 0 1 40 2 0.0 1 0 2 41 1 0.0 1 0 1 42 3 0.0 1 0 3 43 2 0.0 1 1 1 44 1 0.0 1 1 48 3 0.0 1 0 3 49 4 0.0 1 0 4 50 1 0.0 1 0 1 51 3 0.0 1 1 2 52 1 0.0 1 0 1 53 1 0.0 1 0 1 54 3 0.0 1 2 1 55 2 0.0 1 1 1 56 2 0.0 1 0 2 58 2 0.0 1 1 1 59 2 0.0 1 2 60 5 0.0 1 0 5 61 2 0.0 1 1 1 62 1 0.0 1 0 1 63 2 0.0 1 0 2 64 3 0.0 1 2 1 65 2 0.0 1 0 2 66 3 0.0 1 0 3 67 1 0.0 1 0 1 69 3 0.0 1 2 1 70 2 0.0 1 2 71 2 0.0 1 1 1 72 5 0.0 1 2 3 73 1 0.0 1 1 74 4 0.0 1 2 2 75 4 0.0 1 1 3 76 6 0.0 1 4 2 77 7 0.0 1 4 3 78 2 0.0 1 0 2 79 6 0.0 1 2 4 80 3 0.0 1 2 1 83 1 0.0 1 1 84 1 0.0 1 0 1 85 2 0.0 1 0 2 87 2 0.0 1 0 2 88 1 0.0 1 0 1 89 1 0.0 1 0 1 94 2 0.0 1 2 96 1 0.0 1 0 1 97 3 0.0 1 0 3 100 1 0.0 1 0 1 101 1 0.0 1 0 1 105 3 0.0 1 1 2 106 2 0.0 1 0 2 107 1 0.0 1 0 1 111 2 0.0 1 0 2 113 1 0.0 1 0 1 119 1 0.0 1 0 1 120 1 0.0 1 0 1 121 2 0.0 1 0 2 128 1 0.0 1 1 129 1 0.0 1 0 1 130 1 0.0 1 0 1 131 1 0.0 1 0 1 134 1 0.0 1 0 1 136 2 0.0 1 0 2 140 1 0.0 1 0 1 141 1 0.0 1 0 1 147 1 0.0 1 0 1 152 1 0.0 1 0 1 153 1 0.0 1 0 1 158 2 0.0 1 0 2 161 1 0.0 1 0 1 162 2 0.0 1 2 163 1 0.0 1 0 1 164 1 0.0 1 0 1 166 1 0.0 1 0 1 168 1 0.0 1 0 1 169 2 0.0 1 1 1 170 1 0.0 1 1 171 1 0.0 1 0 1 173 2 0.0 1 0 2 174 1 0.0 1 0 1 176 1 0.0 1 0 1 177 2 0.0 1 0 2 178 4 0.0 1 1 3 182 2 0.0 1 0 2 184 2 0.0 1 0 2 185 1 0.0 1 1 188 1 0.0 1 0 1 190 1 0.0 1 0 1 191 1 0.0 1 0 1 194 1 0.0 1 1 196 2 0.0 1 0 2 198 2 0.0 1 1 1 200 1 0.0 1 0 1 206 1 0.0 1 0 1 207 1 0.0 1 0 1 208 1 0.0 1 0 1 210 2 0.0 1 0 2 213 1 0.0 1 0 1 214 1 0.0 1 0 1 215 1 0.0 1 0 1 217 1 0.0 1 0 1 220 1 0.0 1 0 1 223 1 0.0 1 0 1 225 2 0.0 1 0 2 231 1 0.0 1 0 1 232 1 0.0 1 0 1 233 1 0.0 1 0 1 235 1 0.0 1 0 1 241 1 0.0 1 0 1 243 2 0.0 1 0 2 RUN STATISTICS FOR INPUT FILE: ERR3932951_1.fastq ============================================= 202812 sequences processed in total