SUMMARISING RUN PARAMETERS ========================== Input filename: ERR3932950_1.fastq Trimming mode: paired-end Trim Galore version: 0.6.4_dev Cutadapt version: 3.5 Number of cores used for trimming: 1 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Using Nextera adapter for trimming (count: 222). Second best hit was Illumina (count: 0) Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 100 bp This is cutadapt 3.5 with Python 3.10.12 Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA ERR3932950_1.fastq Processing reads on 1 core in single-end mode ... Finished in 7.16 s (12 µs/read; 4.86 M reads/minute). === Summary === Total reads processed: 580,175 Reads with adapters: 105,942 (18.3%) Reads written (passing filters): 580,175 (100.0%) Total basepairs processed: 133,640,783 bp Quality-trimmed: 411,933 bp (0.3%) Total written (filtered): 133,031,840 bp (99.5%) === Adapter 1 === Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 105942 times Minimum overlap: 1 No. of allowed errors: 1-9 bp: 0; 10-12 bp: 1 Bases preceding removed adapters: A: 38.5% C: 17.6% G: 15.3% T: 28.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 88842 145043.8 0 88842 2 12014 36260.9 0 12014 3 3394 9065.2 0 3394 4 546 2266.3 0 546 5 112 566.6 0 112 6 65 141.6 0 65 7 10 35.4 0 10 8 5 8.9 0 5 9 12 2.2 0 9 3 10 9 0.6 1 2 7 11 26 0.1 1 7 19 12 14 0.0 1 2 12 13 10 0.0 1 0 10 14 14 0.0 1 2 12 15 16 0.0 1 4 12 16 13 0.0 1 3 10 17 14 0.0 1 4 10 18 14 0.0 1 4 10 19 8 0.0 1 2 6 20 15 0.0 1 2 13 21 15 0.0 1 3 12 22 12 0.0 1 3 9 23 11 0.0 1 2 9 24 11 0.0 1 3 8 25 6 0.0 1 2 4 26 15 0.0 1 2 13 27 11 0.0 1 2 9 28 16 0.0 1 4 12 29 10 0.0 1 4 6 30 9 0.0 1 4 5 31 8 0.0 1 2 6 32 7 0.0 1 0 7 33 12 0.0 1 2 10 34 11 0.0 1 3 8 35 5 0.0 1 0 5 36 3 0.0 1 0 3 37 6 0.0 1 2 4 38 6 0.0 1 0 6 39 14 0.0 1 2 12 40 9 0.0 1 1 8 41 8 0.0 1 4 4 42 10 0.0 1 0 10 43 9 0.0 1 2 7 44 4 0.0 1 1 3 45 6 0.0 1 2 4 46 8 0.0 1 2 6 47 10 0.0 1 2 8 48 8 0.0 1 2 6 49 5 0.0 1 4 1 50 8 0.0 1 3 5 51 12 0.0 1 1 11 52 8 0.0 1 1 7 53 8 0.0 1 3 5 54 6 0.0 1 2 4 55 9 0.0 1 1 8 56 7 0.0 1 2 5 57 9 0.0 1 1 8 58 7 0.0 1 4 3 59 10 0.0 1 4 6 60 5 0.0 1 1 4 61 5 0.0 1 2 3 62 6 0.0 1 2 4 63 3 0.0 1 1 2 64 6 0.0 1 3 3 65 5 0.0 1 3 2 66 6 0.0 1 3 3 67 8 0.0 1 1 7 68 6 0.0 1 1 5 69 8 0.0 1 3 5 70 3 0.0 1 0 3 71 10 0.0 1 5 5 72 8 0.0 1 5 3 73 12 0.0 1 8 4 74 10 0.0 1 1 9 75 7 0.0 1 5 2 76 16 0.0 1 8 8 77 15 0.0 1 6 9 78 4 0.0 1 2 2 79 11 0.0 1 1 10 80 7 0.0 1 1 6 81 2 0.0 1 1 1 82 1 0.0 1 1 83 1 0.0 1 0 1 84 2 0.0 1 0 2 85 4 0.0 1 0 4 86 4 0.0 1 0 4 87 4 0.0 1 1 3 88 2 0.0 1 0 2 89 1 0.0 1 1 91 1 0.0 1 0 1 92 2 0.0 1 0 2 93 6 0.0 1 1 5 94 1 0.0 1 0 1 96 3 0.0 1 0 3 97 11 0.0 1 1 10 98 2 0.0 1 0 2 99 1 0.0 1 0 1 100 2 0.0 1 0 2 101 4 0.0 1 2 2 102 4 0.0 1 0 4 103 2 0.0 1 1 1 104 1 0.0 1 0 1 105 3 0.0 1 1 2 106 4 0.0 1 0 4 108 2 0.0 1 0 2 109 3 0.0 1 0 3 110 1 0.0 1 0 1 111 3 0.0 1 0 3 112 4 0.0 1 1 3 113 1 0.0 1 0 1 114 1 0.0 1 0 1 115 1 0.0 1 0 1 116 2 0.0 1 0 2 117 1 0.0 1 0 1 119 3 0.0 1 0 3 120 3 0.0 1 1 2 121 3 0.0 1 0 3 122 2 0.0 1 1 1 124 1 0.0 1 0 1 127 1 0.0 1 0 1 128 2 0.0 1 0 2 129 2 0.0 1 1 1 130 1 0.0 1 1 131 1 0.0 1 0 1 132 3 0.0 1 1 2 133 1 0.0 1 0 1 134 2 0.0 1 1 1 135 1 0.0 1 0 1 136 4 0.0 1 1 3 138 1 0.0 1 0 1 141 2 0.0 1 0 2 144 1 0.0 1 0 1 145 2 0.0 1 0 2 148 2 0.0 1 0 2 149 2 0.0 1 1 1 150 2 0.0 1 0 2 151 1 0.0 1 0 1 152 2 0.0 1 0 2 153 2 0.0 1 1 1 154 2 0.0 1 0 2 155 1 0.0 1 0 1 156 2 0.0 1 0 2 157 1 0.0 1 0 1 158 1 0.0 1 0 1 159 1 0.0 1 0 1 160 4 0.0 1 1 3 161 1 0.0 1 0 1 162 3 0.0 1 1 2 163 3 0.0 1 1 2 164 2 0.0 1 0 2 165 1 0.0 1 0 1 166 3 0.0 1 0 3 168 2 0.0 1 0 2 169 4 0.0 1 0 4 171 6 0.0 1 1 5 173 1 0.0 1 0 1 174 3 0.0 1 0 3 175 2 0.0 1 0 2 176 2 0.0 1 0 2 177 2 0.0 1 0 2 178 3 0.0 1 0 3 179 2 0.0 1 0 2 180 2 0.0 1 0 2 181 3 0.0 1 1 2 182 1 0.0 1 0 1 183 2 0.0 1 1 1 184 7 0.0 1 0 7 185 1 0.0 1 0 1 188 3 0.0 1 1 2 189 1 0.0 1 0 1 190 1 0.0 1 0 1 191 2 0.0 1 0 2 192 3 0.0 1 1 2 193 1 0.0 1 0 1 196 2 0.0 1 0 2 197 1 0.0 1 0 1 199 3 0.0 1 0 3 200 2 0.0 1 1 1 201 2 0.0 1 0 2 202 2 0.0 1 0 2 204 1 0.0 1 1 206 3 0.0 1 1 2 207 1 0.0 1 0 1 209 2 0.0 1 0 2 210 1 0.0 1 0 1 212 1 0.0 1 0 1 214 1 0.0 1 0 1 215 1 0.0 1 0 1 217 3 0.0 1 1 2 218 1 0.0 1 0 1 221 1 0.0 1 0 1 224 1 0.0 1 0 1 225 2 0.0 1 0 2 226 4 0.0 1 0 4 227 2 0.0 1 0 2 228 1 0.0 1 0 1 233 1 0.0 1 0 1 234 2 0.0 1 0 2 235 2 0.0 1 0 2 236 2 0.0 1 0 2 237 3 0.0 1 0 3 238 2 0.0 1 0 2 240 1 0.0 1 0 1 241 1 0.0 1 0 1 243 5 0.0 1 0 5 244 3 0.0 1 0 3 245 1 0.0 1 0 1 246 1 0.0 1 0 1 247 1 0.0 1 0 1 248 1 0.0 1 0 1 249 1 0.0 1 0 1 250 1 0.0 1 0 1 RUN STATISTICS FOR INPUT FILE: ERR3932950_1.fastq ============================================= 580175 sequences processed in total